Surprisingly high stability of barley lipid transfer protein, LTP1, towards denaturant, heat and proteases
نویسندگان
چکیده
منابع مشابه
Structural Changes in Barley Protein LTP1 Isoforms at Air-Water Interfaces.
We use a coarse-grained model to study the conformational changes in two barley proteins, LTP1 and its ligand adduct isoform LTP1b, that result from their adsorption to the air-water interface. The model introduces the interface through hydropathy indices. We justify the model by all-atom simulations. The choice of the proteins is motivated by making attempts to understand formation and stabili...
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پروتئین های ناقل لیپید (ltps) گروهی از پروتئین ها با وزن ملکولی متوسط در نزد گیاهان هستند که دارای پتانسیل های کاربردی به ویژه در سیستم های رها سازی دارو می باشند. هدف از این تحقیق، کلون کردن ژن ltp2 مربوط به گیاه برنج(oryza sativa) در وکتور بیانی pgex-6p-2، در باکتری e.coli (top10) جهت تولید پروتئین به صورت متصل با gst می باشد. از آنجائیکه ژن ltp2 در گیاه برنج را فاقد اینترون است این ژن با کمک...
15 صفحه اولEnhanced tolerance to bacterial pathogens caused by the transgenic expression of barley lipid transfer protein LTP2.
Purified lipid transfer protein LTP2 from barley applied on tobacco leaves eliminated symptoms caused by infiltration of Pseudomonas syringae pv. tabaci 153. Growth of the pathogen in leaves of transgenic tobacco plants was retarded when compared with non-transformed controls. The percentage of inoculation points that showed necrotic lesions was greatly reduced in transgenic tobacco (17-38% ver...
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Non-specific lipid transfer protein (LTP) in barley grain reacted with the IgE in 26 sera drawn from food allergy patients. A sandwich-type of enzyme-linked immunosorbent 27 assay (ELISA) was developed with mouse monoclonal antibodies raised against LTP 28 purified with barley flour. This ELISA showed a practical working range of 0.3–3 ng/mL 29 and no cross-reactivity with wheat, adlay and rye....
متن کاملA lipid transfer protein that transfers lipid
Very few lipid transfer proteins (LTPs) have been caught in the act of transferring lipids in vivo from a donor membrane to an acceptor membrane. Now, two studies (Halter, D., S. Neumann, S.M. van Dijk, J. Wolthoorn, A.M. de Maziere, O.V. Vieira, P. Mattjus, J. Klumperman, G. van Meer, and H. Sprong. 2007. J. Cell Biol. 179:101-115; D'Angelo, G., E. Polishchuk, G.D. Tullio, M. Santoro, A.D. Cam...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2001
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(00)02424-8